Mpox Virus Isolate: USA/MA001/2022 Culture Fluid

Mpox virus heat inactivated culture fluid (Isolate: USA/MA001/2022) is an enveloped double‐stranded DNA virus with a genome size of around 190 kb. It belongs to the Orthopoxvirus genus of the Poxviridae family. Mpox virus, hMPXV/USA/MA001/2022 was isolated from a human in Massachusetts, USA in May of 2022, during an outbreak of mpox.1 Mpox virus, hMPXV/USA/MA001/2022 belongs to Clade IIb (previously west African clade) and lineage B.1.2 The complete genome of monkeypox virus, hMPXV/USA/MA001/2022 has been sequenced (GenBank: ON563414.3 and GISAID: EPI_ISL_13052289).

Each frozen aliquot contains 1 mL of heat inactivated viral culture fluid. The pre-inactivation titer was determined from an infectious aliquot.

Viral inactivation is verified after heat inactivation by the absence of viral growth in tissue culture-based infectivity assays.