Endotoxin-Free Utrapure Plasmid Extraction Midiprep System
GDN1001Contact us for more information.
The Endotoxin-Free Utrapure Plasmid Extraction Midiprep System is composed of macroporous silica–based resin with a high density of diethylaminoethyl (DEAE) group, which is developed exclusively for isolation of nucleic acids.Purification is based on the interaction between negatively charged phosphates of nucleic acid backbones and positively charged DEAE groups on the surface of the resin. An innovative endotoxin-removal reagent, E2 reagent, can effectively remove lipopolysaccharides from bacterial cell lysates by phase partition without chilling step. Simply add the E2 reagent into the clear cell lysate, and the lipopolysaccharide contaminants can be removed with the flow-through. By testing with the endotoxin LAL test kit, the sytem consistently keeps the endotoxin level less than 0.1 EU/ug DNA in the final plasmid DNA product.
Contains a kit of 25 preps
GDN1001_Protocol.pdf
