NEPHRINES, PLASMA FASTRACK RIA, 2 X 100 TESTS

First, the plasma proteins are removed by precipitation. Then the metanephrine (metadrenaline) and normetanephrine (normetadrenaline) are quantitatively acylated. The assay procedure follows the basic principle of radioimmunoassay, involving competition between a radioactive and a non-radioactive antigen for a fixed number of antibody binding sites. The amount of 125I-labelled antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the antibody bound radioactivity is precipitated with a second antibody in the presence of polyethylene glycol. The precipitate is counted in a gamma counter. Quantification of unknown samples is achieved by comparing their activity with a reference curve prepared with known standards.

Catalog #: KIPL1400
Format: RIA
Label: I125
Size: 2 x 100 tests
Sample Type: EDTA Plasma
Sample Volume: 200 µL
Controls: 2 levels
Range: see Metanephrine and Normetanephrine Plasma RIA
Sensitivity: see Metanephrine and Normetanephrine Plasma RIA
Incubation: see Metanephrine and Normetanephrine Plasma RIA
Shelf Life (weeks): 14