GLUCAGON, RIA, 100 TESTS

Glucagon in plasma is assayed by the competitive radioimmunoassay using a rabbit antiserum raised against a glucagon-albumin conjugate. Glucagon in standards and samples compete with 125I-labelled glucagon in binding to the antibodies in a two steps incubation. 125I -glucagon binds in a reverse proportion to the concentration of glucagon in standards and samples . Antibody-bound 125I -glucagon is separated from the unbound fraction using double antibody solid phase. The radioactivity of the bound fraction is measured in a gamma counter. The antiserum used in this assay shows less than 0.1% cross reaction with gut-GLI (14). For professional use within a laboratory.

Catalog #: RB310
Format: RIA
Label: I125
Size: 100 tests
Sample Type: Serum
Sample Volume: 200 µL
Controls: 2 levels
Range: 4,7-150 pmol/L
Sensitivity: 3 pmol/L
Incubation: 48h
Shelf Life (weeks): 11