LSP ERBB2 FISH Probe

The ERBB2 FISH Probe is designed to detect the human ERBB2 gene located on chromosome band 17q12. ERBB2 is also known as NEU, NGL, HER2,TKR1, CD340, HER-2, MLN 19 or HER-2/neu.

The ERBB2 FISH probe is labeled with CytoOrange. CytoOrange is a fluorophore with an excitation peak at 551nm and emission peak at 575nm, giving it an orange fluorescent signal with the appropriate filters.

The ERBB2 FISH probe hybridizes to chromosome 17 in both metaphase and interphase cells. After hybridizing with normal human peripheral blood lymphocyte samples, two distinct orange fluorescent spots are observed in the interphase nuclei, each spot corresponding to the 17q12 region on the chromosome 17. In metaphase cells, a “doublet” or “split signal” may appear on chromosomal region 17q12. Each signal within the doublet represents one of the two sister chromatids present in the replicated chromosome 17.

For Research Use Only.